GAD65 Peptide
Product Specifications
NCBI Gene ID
2572
Swiss Prot
Q05329
Accession Number
NP_000809.1
Type
Peptides
Buffer
GAD65 peptide is supplied as 100 μ g of dried peptide. When the peptide is reconstituted in 200 μ L water, the concentration will be 0.5 mg/mL.
Shipping Conditions
Room Temp
Storage Conditions
Store at -20˚ C. When reconstituted minimize freezing and thawing.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=45-648P
Applications Notes
GAD65 Peptide is used for blocking the activity of GAD65 antibody (catalog number 45-648) . The GAD65 antibody ships at 0.5 mg/mL and the GAD65 Peptide ships as a 100 ug pellet. When peptide is reconstituted in 200 uL water, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed) . Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
NCBI Official Name
Glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
NCBI Organism
Homo sapiens
Other Product Names
GAD65, GAD65, Glutamate decarboxylase 2, 65 kDa glutamic acid decarboxylase, GAD-65
Tested Applications
Blocking
Species
Human
Protein ID
4503875
Physical Properties
Lyophilized
Curated Selection
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