NUMB Peptide

Product Specifications

NCBI Gene ID

8650

Swiss Prot

P49757

Accession Number

NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1

Type

Peptides

Buffer

NUMB peptide is supplied as 100 μ g of dried peptide. When the peptide is reconstituted in 200 μ L water, the concentration will be 0.5 mg/mL.

Shipping Conditions

Room Temp

Storage Conditions

Store at -20˚ C. When reconstituted minimize freezing and thawing.

Applications Notes

NUMB Peptide is used for blocking the activity of NUMB antibody (catalog number 45-101) . The NUMB antibody ships at 0.5 mg/mL and the NUMB Peptide ships as a 100 ug pellet. When peptide is reconstituted in 200 uL water, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed) . Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.