GAPDH Peptide
Product Specifications
NCBI Gene ID
2597
Swiss Prot
P04406
Accession Number
NP_002037.2
Type
Peptides
Buffer
GAPDH peptide is supplied as 100 μ g of dried peptide. When the peptide is reconstituted in 200 μ L water, the concentration will be 0.5 mg/mL.
Shipping Conditions
Room Temp
Storage Conditions
Store at -20˚ C. When reconstituted minimize freezing and thawing.
Applications Notes
GAPDH Peptide is used for blocking the activity of GAPDH antibody (catalog number 45-061) . The GAPDH antibody ships at 0.5 mg/mL and the GAPDH Peptide ships as a 100 ug pellet. When peptide is reconstituted in 200 uL water, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed) . Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
NCBI Official Name
Glyceraldehyde-3-phosphate dehydrogenase
NCBI Organism
Homo sapiens
Other Product Names
G3PD, GAPD, HEL-S-162eP, CDABP0047, OK/SW-cl.12, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH, GAPDH
Tested Applications
Blocking
Species
Human
Protein ID
7669492
Physical Properties
Lyophilized
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