PAX3 Peptide

Product Specifications

NCBI Gene ID

5077

Swiss Prot

P23760

Accession Number

NP_852122.1; NP_000429.2; NP_039230.1; NP_852123.1; NP_852124.1; NP_852126.1; NP_852125.1; NP_001120838.1

Type

Peptides

Buffer

PAX3 peptide is supplied as 100 μ g of dried peptide. When the peptide is reconstituted in 200 μ L water, the concentration will be 0.5 mg/mL.

Shipping Conditions

Room Temp

Storage Conditions

Store at -20˚ C. When reconstituted minimize freezing and thawing.

Applications Notes

PAX3 Peptide is used for blocking the activity of PAX3 Antibody (catalog number 42-977) . PAX3 antibody ships at 0.5 mg/mL and the IRF4 Peptide ships as a 100 μ g pellet. When peptide is reconstituted in 200 μ L water, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed) . Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.