5,5′-Dithiobis (2-nitrobenzoic Acid) DTNB

<strong>5,5′-Dithiobis(2-nitrobenzoic Acid) DTNB</strong>_x000D_ <strong>Catalog number:</strong> B2010677_x000D_ <strong>Lot number:</strong> Batch Dependent_x000D_ <strong>Expiration Date:</strong> Batch dependent_x000D_ <strong>Amount:</strong> 1 g_x000D_ <strong>Molecular Weight or Concentration:</strong> 396.35 g/mol_x000D_ <strong>Supplied as:</strong> Powder_x000D_ <strong>Applications:</strong> molecular tool for the study of thiol-containing molecules_x000D_ <strong>Storage:</strong> RT_x000D_ <strong>Keywords:</strong> DTNB, Bis(3-carboxy-4-nitrophenyl) disulfide, Ellman’s Reagent, 3-Carboxy-4-nitrophenyl disulfide, 6,6′-Dinitro-3,3′-dithiodibenzoic acid_x000D_ <strong>Grade:</strong> Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered through 0.22 um._x000D_ _x000D_ <strong>References:</strong>_x000D_ 1: Owen JB, Butterfield DA. Measurement of oxidized/reduced glutathione ratio Methods Mol Biol. 2010;648:269-77._x000D_ 2: Sullivan ML, Bonawitz ND. Spectrophotometric determination of reaction rates and kinetic parameters of a BAHD acyltransferase using DTNB (5,5'-dithio-bis-[2-nitrobenzoic acid]) Plant Sci. 2018 Apr;269:148-152._x000D_ 3: Xing B, Shi Y, Tang W. Kinetic studies of the reactions of some metal reconstituted metallothioneins with the electrophilic disulfide 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) Biometals. 2000 Dec;13(4):295-300._x000D_ 4: Lara HH, Ixtepan-Turrent L, Garza-Treviño EN, Flores-Teviño SM, Borkow G, Rodriguez-Padilla C. Antiviral propierties of 5,5'-dithiobis-2-nitrobenzoic acid and bacitracin against T-tropic human immunodeficiency virus type 1 Virol J. 2011 Mar 24;8:137._x000D_ 5: Huang BH, Shen SS, Wei N, Guo XF, Wang H. Fluorescence biosensor based on silicon quantum dots and 5,5'-dithiobis-(2-nitrobenzoic acid) for thiols in living cells Spectrochim Acta A Mol Biomol Spectrosc. 2020 Mar 15;229:117972._x000D_ 6: Takeda K, Shigemura A, Hamada S, Gu W, Fang D, Sasa K, Hachiya K. Dependence of reaction rate of 5,5'-dithiobis-(2-nitrobenzoic acid) to free sulfhydryl groups of bovine serum albumin and ovalbumin on the protein conformations J Protein Chem. 1992 Apr;11(2):187-92._x000D_ 7: Reithmeier RA. Inhibition of anion transport in human red blood cells by 5,5'-dithiobis(2-nitrobenzoic acid) Biochim Biophys Acta. 1983 Jul 13;732(1):122-5._x000D_ 8: Li ZG. Quantification of hydrogen sulfide concentration using methylene blue and 5,5'-dithiobis(2-nitrobenzoic acid) methods in plants Methods Enzymol. 2015;554:101-10._x000D_ 9: Chen W, Zhao Y, Seefeldt T, Guan X. Determination of thiols and disulfides via HPLC quantification of 5-thio-2-nitrobenzoic acid J Pharm Biomed Anal. 2008 Dec 15;48(5):1375-80._x000D_ <a href="https://pubmed.ncbi.nlm.nih.gov/6872259">10: Ghiggeri GM, Candiano G, Delfino G, Queirolo C. A modification of the 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) method for the determination of the sulphhydryl content of human serum albumin Clin Chim Acta. 1983 May 30;130(2):257-61. </a>_x000D_ _x000D_ <strong>Products Related to 5,5′-Dithiobis(2-nitrobenzoic Acid) DTNB</strong><a href="https://moleculardepot.com/product-category/Chemicals/">: Chemicals</a>