Siglec-10 Recombinant Protein

Product Specifications

Background

The siglecs (sialic acid-binding Ig-like lectins) are a distinct subset of the Ig superfamily with adhesion-molecule-like structure. We describe here a novel member of the siglec protein family that shares a similar structure including five Ig-like domains, a transmembrane domain, and a cytoplasmic tail containing two ITIM-signaling motifs. Siglec-10 was identified through database mining of an asthmatic eosinophil EST library. The Siglec-10-VAP-1 interaction seems to mediate lymphocyte adhesion to endothelium and has the potential to modify the inflammatory microenvironment via the enzymatic end products.

CAS Number

9000-83-3

NCBI Gene ID

89790

Swiss Prot

Q96LC7

Accession Number

NP_149121.2

Tag

His Tag

Type

Recombinant Proteins

Source

HEK293 cells

Purity

>95% as determined by SDS-PAGE.

Buffer

25 mM MES, 150 mM NaCl, Arginine, pH5.5

Shipping Conditions

Room Temp

Storage Conditions

Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

60.1 kDa

Applications Notes

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 60.1 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.