Integrin alpha 4 Recombinant Protein

The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains (reviewed in 1) . Alpha 4 (4) chain associates with either beta 1 (1) or beta 77) chain. It has been demonstrated that the putative ligand-binding sites of both integrin 41 and 47 is located on the 4 chain. These ligands included Madcam, VCAM, and fibronectin (2-4) . Madcam is known as the principal ligand for integrin a4b7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin 47, the gut mucosal homing receptor for peripheral T cells (5) . This recombinant protein fragment includes the ligand and monoclonal antibody binding domains; this binding has been tested and verified in ELISA assays. This recombinant protein is recognized PSI antibodies Integrin 4 (IN1) (PSI 4827) and Integrin 4 (IN2) (PSI 4837), but not Integrin 4 (CT) (PSI 4783) .

Store in working aliquots at -70˚ C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Wegener KL and Campbell ID. Transmembrane and cytoplasmic domains in integrin activation and protein-protein interactions. Mol. Membr. Biol. 2008; 25:376-87.

Kamata T, Puzon W, and Takada Y. Identification of putative ligand-binding sites of the integrin alpha 4 beta 1 (VLA-4, CD49d/CD29) . Biochem J. 1995; 305:945-51.

Berlin C, Berg EL, Briskin MJ, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 1993; 74:185-95.

Pulido R, Elices MJ, Campanero MR, et al. Functional evidence for three distinct and independently inhibitable adhesion activities mediated by the human integrin VLA-4. Correlation with distinct alpha 4 epitopes. J. Biol. Chem. 1991; 266:10241-5.