LGR4 / GPR48 Recombinant Protein

Product Specifications

Background

The fourth member of the leucine-rich repeat-containing GPCR family (LGR4, frequently referred to as GPR48) together with its family members LGR5 and -6, bind to R-spondins (RSPOs) -1–4 and result in Wnt signaling potentiation. In addition, LGR4 (as well as LGR5 and -6) is implicated in multiple cancers and promotes invasion and metastasis in colorectal, prostate, and cervical cancer cell lines.

CAS Number

9000-83-3

NCBI Gene ID

55366

Swiss Prot

Q9BXB1

Accession Number

NP_060960.2

Tag

His Tag

Type

Recombinant Proteins

Source

HEK293 cells

Purity

>85% as determined by SDS-PAGE.

Buffer

PBS, pH7.4

Shipping Conditions

Room Temp

Storage Conditions

Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

58.8 kDa

Applications Notes

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 58.8 kDa. The protein migrates as 66-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

NCBI Official Name

LeucinerichrepeatcontainingGprotein-coupledreceptor4

NCBI Organism

Homo sapiens

Background Reference 01

Yue Z. et al. FASEB J. 2018. 32 (5) :2422-2437.

Background Reference 02

Gao Y. et al. Up-regulation of GPR48 induced by down-regulation of p27Kip1 enhances carcinoma cell invasiveness and metastasis. Cancer Res. 2006 Dec 15; 66 (24) :11623-31.