Rhesus macaque CD28 Recombinant Protein

Product Specifications

Background

T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2) . When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs) . The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

CAS Number

9000-83-3

NCBI Gene ID

940

Swiss Prot

P10747

Accession Number

NP_006130.1

Tag

Mouse IgG Fc Tag

Type

Recombinant Proteins

Source

HEK293 cells

Field of Research

Homeostasis, Immunology, Obesity, Signal Transduction, Stem Cell

Purity

>95% as determined by SDS-PAGE.

Buffer

50 mM Tris, 100 mM Glycine, pH7.5

Shipping Conditions

Room Temp

Storage Conditions

Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

42.0 kDa

Applications Notes

This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 42.0 kDa. The protein migrates as 50-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.