Streptavidin Recombinant Protein
Product Specifications
Background
Streptavidin is a tetrameric protein purified from the bacterium Streptomyces avidinii, and exhibits high binding affinity for biotin. Able to bind one molecule of biotin with each subunit. Streptavidin (PI=6.0-7.5) has lower level of non-specific binding to various biological components at physiological pH than avidin (PI=7.4), resulting from its isoelectric point (PI) .Streptavidin is useful in affinity chromatography, ELISA, immunohistochemistry and Western Blotting.
Type
Recombinant Proteins
Source
E. coli
Buffer
NaCl, pH7.4
Shipping Conditions
Room Temp
Storage Conditions
Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=11-220
Applications Notes
This protein .
Background Reference 01
Green, NM., 1975, Advances in protein chemistry 29: 85–133.
Background Reference 02
Hendrickson, W. A., 1989, Proceedings of the National Academy of Sciences 86 (7) : 2190–4.
Background Reference 03
Chivers Claire E., et al., 2010, Nature Methods 7 (5) : 391–3.
Background Reference 04
Osojic GN., et al., 2012, Small 8 (12) : 1840–5.
Other Product Names
Streptavidin, SA
Tested Applications
ELISA, WB
Physical Properties
Lyophilized
CAS Number
9000-83-3
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