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Protein G Recombinant Protein

Product Specifications

Background

Protein G is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria much like Protein A but with differing specificities. It is a 65-kDa (G148 protein G) and a 58 kDa (C40 protein G) cell surface protein that has found application in purifying antibodies through its binding to the Fc region. The native molecule also binds albumin, however, because serum albumin is a major contaminant of antibody sources, the albumin binding site has been removed from this recombinant form of Protein G.

CAS Number

9000-83-3

Swiss Prot

P19909

Tag

His Tag

Type

Recombinant Proteins

Source

E. coli

Purity

>98% as determined by SDS-PAGE.

Buffer

0.1 M NaH2PO4, pH8.5

Shipping Conditions

Room Temp

Storage Conditions

Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

Applications Notes

This recombinant protein can be used for WB. For research use only.

Background Reference 01

Sjobring U, Bjorck L, Kastern W, et al., 1991, J Biol Chem 266 : 399–405.

Other Product Names

RPG, Protein G

Tested Applications

WB

Species

Others

Physical Properties

Lyophilized

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