Protein G Recombinant Protein
Product Specifications
Background
Protein G is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria much like Protein A but with differing specificities. It is a 65-kDa (G148 protein G) and a 58 kDa (C40 protein G) cell surface protein that has found application in purifying antibodies through its binding to the Fc region. The native molecule also binds albumin, however, because serum albumin is a major contaminant of antibody sources, the albumin binding site has been removed from this recombinant form of Protein G.
CAS Number
9000-83-3
Swiss Prot
P19909
Tag
His Tag
Type
Recombinant Proteins
Source
E. coli
Purity
>98% as determined by SDS-PAGE.
Buffer
0.1 M NaH2PO4, pH8.5
Shipping Conditions
Room Temp
Storage Conditions
Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.
Applications Notes
This recombinant protein can be used for WB. For research use only.
Background Reference 01
Sjobring U, Bjorck L, Kastern W, et al., 1991, J Biol Chem 266 : 399–405.
Other Product Names
RPG, Protein G
Tested Applications
WB
Species
Others
Physical Properties
Lyophilized
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