PSMA / FOLH1 Recombinant Protein
Product Specifications
Background
Prostate-specific membrane antigen (PSMA) is also known as Folate hydrolase 1 (FOLH1), Glutamate carboxypeptidase 2 (GCP2), N-acetylated-alpha-linked acidic dipeptidase I (NAALAD1), which belongs to the peptidase M28 family and M28B subfamily. FOLH1 / PSMA is stable at pH greater than 6.5. FOLH1 / PSMA is a type II transmembrane zinc metallopeptidase that is most highly expressed in the nervous system, prostate, kidney, and small intestine. FOLH1 / GCP-2 is homodimer and binds 2 zinc ions per subunit, and required for NAALADase activity. The catalytic activity of PSMA involved in releasing of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly – gamma - glutamates. FOLH1 / GCP-2 / PSMA has both folate hydrolase and N – acetylated – alpha – linked - acidic dipeptidase (NAALADase) activity and has a preference for tri-alpha-glutamate peptides. GCP-2 / PSMA involved in prostate tumor progression and also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.
NCBI Gene ID
53320
Swiss Prot
O35409
Accession Number
NP_058050.3
Tag
Fc Tag
Type
Recombinant Proteins
Source
HEK293 cells
Purity
>95% as determined by SDS-PAGE.
Buffer
50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5
Shipping Conditions
Room Temp
Storage Conditions
Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=11-228
Calculated Molecular Weight
106.0 kDa
Applications Notes
This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 106.0 kDa. The protein migrates as 110-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
NCBI Official Name
Folatehydrolase1
NCBI Organism
Mus musculus
Background Reference 01
Grauer L.S., et al., 1998, Cancer Res. 58:4787-4789.
Background Reference 02
Mesters J.R., et al., 2006, EMBO J. 25:1375-1384.
Background Reference 03
Barinka C., et al., 2009, J. Med. Chem. 50:3267-3273.
Other Product Names
FOLH1, PSMA, GIG27, FOLH, NAALAD1, PSM, NAALADase I, GCPII, FGCP
Tested Applications
ELISA, WB
Species
Human
Physical Properties
Lyophilized
CAS Number
9000-83-3
Curated Selection
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