B7-H5 / Gi24 / VISTA Recombinant Protein

Product Specifications

Background

Platelet receptor Gi24, also known as B7-H5 and stress-induced secreted protein-1 (Sisp-1), is a protein that in humans is encoded by the C10orf54 gene, which contains 1 Ig-like (immunoglobulin-like) domain. As for C10orf54 gene, C10orf54 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state on ESCs. Human C10orf54 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment.

CAS Number

9000-83-3

NCBI Gene ID

64115

Swiss Prot

Q9H7M9

Accession Number

AAH20568.1

Tag

Mouse IgG Fc Tag

Type

Recombinant Proteins

Source

HEK293 cells

Field of Research

Immunology

Purity

>95% as determined by SDS-PAGE.

Buffer

50 mM Tris, 100 mM Glycine, pH7.5

Shipping Conditions

Room Temp

Storage Conditions

Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

45.0 kDa

Applications Notes

This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 45.0 kDa. The protein migrates as 55-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.