IRAK Recombinant Protein

Nuclear factor kappa B (NF-B) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-B mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNF and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently (1,2) . IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response (3) . IRAK mediates a signaling cascade leading to NF-B activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF) (1,4) .

Store in working aliquots at -70˚ C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Trofimova M, Sprenkle AB, Green M; et al. Developmental and tissue-specific expression of mouse pelle-like protein kinase. J. Bio. Chem. 1996; 271: 17609-12.

Jianing Huang, Xiong Gao, Shyun Li, et al. Recruitment of IRAK to the interleukin 1 receptor complex requires interleukin-1 receptor accessory protein. Proc. Natl. Acad. Sci. USA 1997; 94:12829-32.

Robinson D, Shibuya K, Mui A, et al. IGIF does not drive Th1 development but synergizes with IL-12 for interferon-gamma production and activates IRAK and NF-kB. Immunity 1997; 7:571-81.