NKG2D / CD314 Recombinant Protein

Product Specifications

Background

NKG2D is a transmembrane protein belonging to the CD94/NKG2 family of C-type lectin-like receptors, also known as KLRK1, CD314, D12S2489E, KLR and killer cell lectin like receptor K1. NKG2D itself forms a homodimer whose ectodomains serve for ligand binding. NKG2D is a major recognition receptor for the detection and elimination of transformed and infected cells as its ligands are induced during cellular stress, either as a result of infection or genomic stress such as in cancer. In NK cells, NKG2D serves as an activating receptor, which itself is able to trigger cytotoxicity. The function of NKG2D on CD8+ T cells is to send co-stimulatory signals to activate them.

CAS Number

9000-83-3

NCBI Gene ID

22914

Swiss Prot

P26718

Accession Number

NP_031386.2 (T72A)

Tag

Fc Tag

Type

Recombinant Proteins

Source

HEK293 cells

Purity

>90% as determined by SDS-PAGE.

Buffer

50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5

Shipping Conditions

Room Temp

Storage Conditions

Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

43.1 kDa

Applications Notes

This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 43.1 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.