Neutrophil cytosol factor 1 Recombinant Protein

Neutrophil cytosol factor 1 (NCF1) is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is characterized as a multicomponent enzyme which is activated to produce superoxide anion. NCF2, NCF1, and a membrane bound cytochrome b558 are required for the activation of the latent NADPH oxidase. The human NCF1 gene encodes a 390 amino acids protein without a signal peptide. The NCF1 gene interacts with other subunits of nicotinamide adenine dinucleotide phosphate-oxidase (NADPH) and plays an important role in innate immunity, producing reactive oxygen species and reducing the severity and duration of parasitic infection and autoimmune disease. NCF1 also has a role in T cell activation.

Lyophilized from a 0.2 um filtered solution of 20mM Tris pH8.0,1mM DTT,0.1M NaCl. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O.

NCF-1/47 kDa autosomal chronic granulomatous disease protein/47 kDa neutrophil oxidase factor/NCF-47K/Neutrophil NADPH oxidase factor 1/Nox organizer 2/Nox-organizing protein 2/SH3 and PX domain-containing protein 1A/p47-phox, NCF1