LGR5 / GPR49 Recombinant Protein

Product Specifications

Background

LGR5 (also known as GPR49) is a seven-transmembrane protein of the class A Rhodopsin-like family of GPCRs. LGR5 and LGR4 bind the R-spondins with high affinity and mediate the potentiation of Wnt/β-catenin signaling by enhancing Wnt-induced LRP6 phosphorylation. The LGR5/RSPO complex could promote Wnt signalling via the neutralization of two transmembrane E3 ligases, RNF43 and ZNRF3.

CAS Number

9000-83-3

NCBI Gene ID

8549

Swiss Prot

O75473

Accession Number

NP_003658.1

Tag

His Tag

Type

Recombinant Proteins

Source

HEK293 cells

Purity

>90% as determined by SDS-PAGE.

Buffer

PBS, pH7.4

Shipping Conditions

Room Temp

Storage Conditions

Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

62.0 kDa

Applications Notes

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 62.0 kDa. The protein migrates as 66-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

NCBI Official Name

LeucinerichrepeatcontainingGprotein-coupledreceptor5

NCBI Organism

Homo sapiens

Background Reference 01

Morgan RG. et al. Br J Cancer. 2018 May 29; 118 (11) : 1410–1418.

Background Reference 02

Yue Z. et al. FASEB J. 2018 May;32 (5) :2422-2437.

Background Reference 03

De Lau W. et al. Genes & development. 2014;28:305–16.

Other Product Names

LGR5, GPR49, GPR67, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38, Leucine-rich repeat-containing G-protein coupled receptor 5