IL-8 / CXCL8 Recombinant Protein

Product Specifications

Background

Interleukin 8 (IL8 or chemokine (C-X-C motif) ligand 8, CXCL8) is a chemokine produced by macrophages and other cell types such as epithelial cells, airway smooth muscle cells and endothelial cells. There are many receptors on the surface membrane capable of binding IL-8, the most frequently studied types are the G protein-coupled serpentine receptors CXCR1 and CXCR2. Expression and affinity for IL-8 differs between the two receptors (CXCR1 > CXCR2) . IL-8 (6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8 (5-77) has increased activity on neutrophil activation and IL-8 (7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8 (1-77), respectively.

CAS Number

9000-83-3

NCBI Gene ID

3576

Swiss Prot

P10145

Accession Number

NP_000575.1

Tag

Fc Tag

Type

Recombinant Proteins

Source

HEK293 cells

Purity

>95% as determined by SDS-PAGE.

Buffer

50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5

Shipping Conditions

Room Temp

Storage Conditions

Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

34.8 kDa

Applications Notes

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 34.8 kDa. The protein migrates as 37-43 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.