IL-33 Recombinant Protein

Product Specifications

Background

Interleukin 33 (IL33) is known as C9orf26, DKFZp586H0523, DVS27, NF-HEV, NFEHEV, RP11-575C20.2, and is a cytokine belonging to the IL-1 superfamily. IL-33 induces helper T cells, mast cells, eosinophils and basophils to produce type 2 cytokines. IL-33 mediates its biological effects by interacting with the receptors ST2 (aka IL1RL1) and IL-1 Receptor Accessory Protein (IL1RAP), activating intracellular molecules in the NF-κB and MAP kinase signaling pathways that drive production of type 2 cytokines (e.g. IL-5 and IL-13) from polarized Th2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs.

CAS Number

9000-83-3

NCBI Gene ID

77125

Swiss Prot

Q8BVZ5

Accession Number

NP_598536.2

Tag

His Tag

Type

Recombinant Proteins

Source

HEK293 cells

Purity

>90% as determined by SDS-PAGE.

Buffer

PBS, pH7.4

Shipping Conditions

Room Temp

Storage Conditions

Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

19.5 kDa

Applications Notes

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 19.5 kDa. The protein migrates as 20-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.