GUCY2C / Guanylyl cyclase C Recombinant Protein

Product Specifications

Background

GUCY2C (Guanylyl Cyclase C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (gc) family that signal by producing cGMP. Guanylate cyclase C (GUCY2C) and its hormones guanylin and uroguanylin have recently emerged as one paracrine axis defending intestinal mucosal integrity against mutational, chemical, and inflammatory injury. GUCY2C murine CAR-T cells recognized and killed human colorectal cancer cells endogenously expressing GUCY2C. Thus, we have identified a human GUCY2C-specific CAR-T cell therapy approach that may be developed for the treatment of GUCY2C-expressing metastatic colorectal cancer.

CAS Number

9000-83-3

NCBI Gene ID

2984

Swiss Prot

P25092

Accession Number

NP_004954.2

Tag

Fc Tag

Type

Recombinant Proteins

Source

HEK293 cells

Purity

>95% as determined by SDS-PAGE.

Buffer

50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5

Shipping Conditions

Room Temp

Storage Conditions

Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

72.5 kDa

Applications Notes

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 72.5 kDa. The protein migrates as 80-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.