Flt3-Ligand Recombinant Protein

Flt3-Ligand is a growth factor that regulates proliferation of early hematopoietic cells. Flt3-Ligand binds to cells expressing the tyrosine kinase receptor Flt3. Flt3-Ligand, by itself does not stimulate proliferation of early hematopoietic cells, but synergizes with other CSFs and interleukins to induce growth and differentiation. Unlike SCF, Flt3-Ligand exerts no activity on mast cells. Multiple isoforms of Flt3-Ligand have been identified. The predominant biologically active form is anchored to the cell surface as the extracellular domain of a transmembrane protein (209 a.a.) . The membrane-bound isoform can be proteolytically cleaved to generate a biologically active soluble isoform. Recombinant human Flt3-Ligand is a soluble 17.6 kDa protein consisting of 155 amino acid residues.

The lyophilized Flt3-Ligand recombinant protein is stable for at least 2 years from date of receipt at -20˚ C. Reconstituted Flt3-Ligand is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚ C. As with any protein, exposing Flt3-Ligand recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.