Ebolavirus Glycoprotein Recombinant Protein
Product Specifications
Background
EBOV encodes seven structural proteins: nucleoprotein (NP), polymerase cofactor (VP35), (VP40), GP, transcription activator (VP30), VP24, and RNA polymerase (L) . GP protein contains 160-kDa envelope-attached glycoprotein (GP) and a 110 kDa secreted glycoprotein (sGP) . GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . GP1 is responsible for binding to the receptor (s) on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. GP2 acts as a class I viral fusion protein. GP1,2 mediates endothelial cell activation and decreases endothelial barrier function. sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha.
NCBI Gene ID
279888
Swiss Prot
P87666.1
Accession Number
AAQ55048.1
Tag
His Tag
Type
Recombinant Proteins
Source
HEK293 cells
Field of Research
Infectious Disease
Buffer
PBS, pH7.4
Shipping Conditions
Room Temp
Storage Conditions
Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=97-051
Calculated Molecular Weight
67 kDa
Applications Notes
This recombinant protein can be used for WB. For research use only.
NCBI Official Name
Virion spike glycoprotein precursor
NCBI Organism
Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)
Background Reference 01
Saeed M.F., et al., 2010, PLoS Pathog. 6:0-0.
Background Reference 02
Marzi A., et al., 2006, J. Virol. 80:6305-6317.
Background Reference 03
Chandran K., et al., 2005, Science 308:1643-1645.
Other Product Names
Ebolavirus Glycoprotein
Tested Applications
WB
Species
Ebolavirus
Physical Properties
Lyophilized
CAS Number
9000-83-3
Curated Selection
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