ATG7 Recombinant Protein

Product Specifications

Background

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2) . This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1 (3) . Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme; this function is conserved in the mammalian homolog (4) . In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles (5) . It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death (6) .

CAS Number

9000-83-3

NCBI Gene ID

74244

Swiss Prot

Q9D906

Accession Number

AAH58597

Tag

Fusion Partner:

Type

Recombinant Proteins

Source

E. coli

Field of Research

Autophagy

Purity

~95%

Buffer

1X PBS containing 10% glycerol

Shipping Conditions

Dry Ice

Storage Conditions

Store in working aliquots at -70˚ C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Calculated Molecular Weight

30 kDa (Calculated)

Applications Notes

This recombinant protein can be used for WB and ELISA. For research use only.

NCBI Official Name

Autophagy related 7

NCBI Organism

Mus musculus

Background Reference 01

Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene . 2004; 23:2891-906.

Background Reference 02

Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.