ATG5 Recombinant Protein

Product Specifications

Background

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2) . This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1 (3) . ATG5, another member of the autophagy protein family, forms a conjugate with ATG12; this conjugate has a ubiquitin-protein ligase (E3) -like activity for protein lipidation in autophagy (4) . This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells (5) .

CAS Number

9000-83-3

NCBI Gene ID

9474

Swiss Prot

A9UGY9

Accession Number

AAH02166

Tag

Fusion Partner:

Type

Recombinant Proteins

Source

E. coli

Field of Research

Autophagy, Homeostasis, Innate Immunity, Signal Transduction

Purity

~95%

Buffer

1X PBS containing 10% glycerol

Shipping Conditions

Dry Ice

Storage Conditions

Store in working aliquots at -70˚ C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Calculated Molecular Weight

34 kDa (Calculated)

Applications Notes

This recombinant protein can be used for WB and ELISA. For research use only.

NCBI Official Name

ATG5 autophagy related 5 homolog (S. cerevisiae)

NCBI Organism

Homo sapiens

Background Reference 01

Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene . 2004; 23:2891-906.

Background Reference 02

Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.

Background Reference 03

Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol. 2000; 150:1507-13.

Background Reference 04

Hanada T, Noda NN, Satomi Y, et al. The Atg12-Atg5 conjugate has a novel E3-like activity for protein lipidation in autophagy. J. Biol. Chem. 2007; 282:37298-302.