DR6 Cytoplasmic Domain Recombinant Protein

Product Specifications

Background

Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer (reviewed in 1) . Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNF-R1 and Fas. Several death receptors including DR3, DR4, DR5, and DR6 have been identified. Like TNF-R1, DR6 (also known as TNFRSF21) interacts with death domain containing adapter molecule TRADD. Overexpression of DR6 induces apoptosis and activates NF-kB and JNK. DR6 is widely expressed in human tissues and cell lines (2) . Recently, DR6 was found to interact with an amino-terminal fragment of the Beta-amyloid protein (APP) in neurons, activating a caspase 6-dependent apoptotic event leading to axonal degeneration and pruning (3) . The doublet observed in the SDS-PAGE is likely due to incomplete removal of the signal sequence.

CAS Number

9000-83-3

NCBI Gene ID

27242

Swiss Prot

O75509

Accession Number

NP_055267

Tag

Fusion Partner:

Type

Recombinant Proteins

Source

E. coli

Field of Research

Apoptosis

Purity

~95%

Buffer

1X PBS containing 10% glycerol

Shipping Conditions

Dry Ice

Storage Conditions

Store in working aliquots at -70˚ C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Calculated Molecular Weight

36 kDa (Calculated)

Applications Notes

This recombinant protein can be used for WB and ELISA. For research use only.

NCBI Official Name

Tumor necrosis factor receptor superfamily, member 21

NCBI Organism

Homo sapiens

Background Reference 01

Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.

Background Reference 02

Pan G, Bauer JH, Haridas V, et al. Identification and functional characterization of DR6, a novel death domain-containing TNF receptor. FEBS Lett 1998; 431:351-6.