Goat Anti-Rat IgG (H&L) Antibody (Alkaline Phosphatase)

Alkaline Phosphatase Conjugated Affinity Purified anti-Rat IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against Human Serum Proteins.

0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0; 0.01% (w/v) Sodium Azide; 10 mg/mL Bovine Serum Albumin (BSA)

Alkaline Phosphatase Conjugated Affinity Purified anti-Rat IgG can be stored vial at 4 ˚ C before opening. DO NOT FREEZE. This product is stable at 4 ˚ C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.

Alkaline Phosphatase Conjugated Affinity Purified anti-Rat IgG is suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended dilutions are as follows, ELISA 1:4,000 - 1:20,000, Western Blot 1:1,000 - 1:5,000, Immunohistochemistry 1:200 - 1:1,000 . (Optimal dilution should be determined by user) .