Rabbit Anti-Goat IgG (H&L) Antibody (HRP)

Peroxidase Conjugated Affinity Purified anti-Goat IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against Human Serum Proteins.

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2; 10 mg/mL Bovine Serum Albumin (BSA) ; 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Peroxidase Conjugated Affinity Purified anti-Goat IgG can be stored vial at 4 ˚ C prior to restoration. Resuspend with 1.0 ml of deionized water (or equivalent. For extended storage aliquot contents and freeze at -20 ˚ C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 ˚ C as an undiluted liquid. Dilute only prior to immediate use.

Peroxidase Conjugated Affinity Purified anti-Goat IgG is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended dilutions are as follows, ELISA 1:14,000 - 1:60,000, Western Blot 1:1,000 - 1:5,000, Immunohistochemistry 1:500 - 1:2,500. (Optimal dilution should be determined by user) .