Recombinant Human Interleukin-33 protein (IL33) (Active)

Cytokine that binds to and signals through the IL1RL1, ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:16286016) . Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury (PubMed:17853410, PubMed:18836528) . {ECO:0000269|PubMed:16286016, ECO:0000269|PubMed:17853410, ECO:0000269|PubMed:18836528}.; In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB, RELA, lowering expression of its targets (PubMed:21734074) . This form is rapidely lost upon angiogenic or proinflammatory activation (PubMed:18787100) . {ECO:0000269|PubMed:18787100, ECO:0000269|PubMed:21734074}.

SITGISPIT EYLASLSTYN DQSITFALED ESYEIYVEDL KKDEKKDKVLLSYYESQHPS NE SGDGVDGK MLMVTLSPTK DFWLHANNKE HSVELHKCEKPLPDQAFFVL HNMHSNCVSF ECKTDPGVFI GVKDNHLALI KVDSSENLCT ENILFKLSET

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 0.05 ng, ml, corresponding to a specific activity of 2.0 107 IU, mg.

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.