Human MMP-7 / PUMP1 Protein, His Tag (active enzyme)

Human MMP-7, His Tag (MM7-H5249) is expressed from human 293 cells (HEK293) . It contains AA Leu 18 - Lys 267 (Accession # P09237-1) . It needs to be activated by agents such as APMA in vitro to have hydrolytic activity.

Product Specifications

Background

Matrix metalloproteinase-7 (MMP-7) also known as neutrophil collagenase and CLG1, is a member of matrix metalloproteinases (MMPs) family, which degrade components of the extracellular matrix (ECM) and play essential roles in various physiological processes as well as pathological processes. MMP-7 may affect the metastatic behavior of breast cancer cells through protection against lymph node metastasis, underlining the importance of anti-target identification in drug development. MMP-7 in the tumor may have a protective effect against lymph node metastasis.

Specifications

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 29.7 kDa. The protein migrates as 30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P09237-1

Expression Region

Leu 18 - Lys 267

Host

HEK293

Target

MMP-7

Conjugation

Unconjugated

Tag

N-10xHis

Source

Human

Stability

The product MUST be stored at -70°C or lower upon receipt; -70°C for 3 months under sterile conditions.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Human MMP-7, His Tag (Cat. No. MM7-H5249) at 1 μg/mL (100 μL/well) can bind various dilution ratio of Anti-MMP7 Polyclonal Antibody (Routinely tested).