HBC

HBC (HBC 530) is a GFP fluorophore-like synthetic dye, with a structurally rigid electron acceptor and a strong electron donor. HBC is nonfluorescent in solution, and when combined with Pepper (RNA aptamer), HBC forms a tight complex and activates and emits bright fluorescence (Kd of ~3.5 nM) . HBC emission peaks vary in different complexes and covers the spectrum from cyan to red. HBC can be used in the live cell imaging of RNA (Em/Ex = 530/485 nm) [1].