Human Ephrin-B2 / EFNB2 Protein, Fc tag (MALS verified)
Human Ephrin-B2 Protein, Fc tag (EPN-H5259) is expressed from human 293 cells (HEK293) . It contains AA Ser 25 - Ala 168 (Accession # P52799-1) .
Product Specifications
Background
Ephrin-B2 (EFNB2) is also known as EPH-related receptor tyrosine kinase ligand 5 (LERK-5) or HTK ligand (HTK-L), which belongs to the ephrin family. The ephrin-B (ENFB) class are transmembrane proteins implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. EFNB2 has been shown to interact with EPHA3 and EPHB1 in optic chiasm development. EFNB2 has also been shown to serve as a receptor for Hendra Virus and Nipah Virus, mediating entry into the cell during infection.
Specifications
This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 42.8 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
P52799-1
Expression Region
Ser 25 - Ala 168
Host
HEK293
Target
Ephrin-B2
Conjugation
Unconjugated
Tag
C-Fc
Source
Human
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
95%
Bioactivity
Immobilized Nipah virus Glycoprotein, His Tag (Cat. No. GLN-N52H3) at 5 μg/mL (100 μL/well) can bind Human Ephrin-B2 Protein, Fc tag (Cat. No. EPN-H5259) with a linear range of 0.1-2 ng/mL (QC tested).
Format
Powder
Buffer
50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5
Additives
Trehalose
Molecular Weight
42.8 kDa
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
500ug*1
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/EPN-H5259.pdf
Species
Human
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