Anti-ENaC beta Antibody

Mouse Monoclonal Antibody specific to ENaC beta

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Beta-NaCH, Epithelial Na(+ channel subunit beta, Beta-ENaC, Nonvoltage-gated sodium channel 1 subunit beta, SCNEB

Gene Name

Scnn1b

NCBI Gene ID

UniProt

P37090

Accession Number

NP_036780

Cellular Locus

Apical cell membrane, Cytoplasmic vesicle membrane

Host

Mouse

Reactivity

Mouse

Immunogen

Synthetic peptide corresponding to C-terminal aa 617-638 of rat ENaC alpha.

Target Antigen

Amiloride-sensitive sodium channel subunit beta

Target

ENaC beta

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

Field of Research

Ion Channels

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride (PubMed:9118951). Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception. {UniProtKB:P51168, PubMed:9118951}.

Additionnal Information

Immunoblotting: use at 1ug/mL. <br><br>Immunohistochemistry: use at 5-10ug/mL. <br><br>Endusers should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

Mouse ENaC beta

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.

Buffer pH

pH 7.4

Target Background

The epithelial sodium channel (ENaC) is a membrane-bound ion-channel that is selectively permeable to Na+ ions and that is assembled as a heterotrimer composed of three homologous subunits alpha, beta, and g. It is involved primarily in the reabsorption of sodium ions in the collecting ducts of the kidney's nephrons. These channels mediate the first step of active sodium reabsorption essential for the maintenance of body salt and water homeostasis. In vertebrates, the channels control reabsorption of sodium in kidney, colon, lung and sweat glands; they also play a role in taste perception.