CD40LG Antibody (Biotin)
Mouse monoclonal antibody against CD40LG conjugated to Biotin
Product Specifications
Product Name Alternative
Anti-CD154 antibody, anti-gp39 antibody, anti-hCD40L antibody, anti-HIGM1 antibody, anti-IGM antibody, anti-IMD3 antibody, anti-T B cell activating molecule antibody, anti-T BAM antibody, anti-T-cell antigen Gp39 antibody, anti-TNF-related activation protein antibody, anti-TrAP antibody, anti-Tumor necrosis factor ligand superfamily member 5 (TNFSF5) antibody
UniProt
P29965
Reactivity
Human
Immunogen
Recombinant fragment (around aa 108-261) of human CD40LG protein (exact sequence is proprietary)
Target
CD40LG
Clonality
Monoclonal
Clone
CD40LG/2761
Conjugation
Biotin
Field of Research
Immunology & Inflammation
Purification
Protein A
Concentration
Purified Ab conjugated to Biotin
Dilution
ELISA (For coating, order antibody without BSA) ; Flow Cytometry (2-4ug/million cells) ; , Immunofluorescence (2-4ug/ml) ; Western Blot (2-4ug/ml) ; , Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT), (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Form
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Molecular Weight
36kDa
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Jurkat cells. Tonsil, Spleen or Thymus
Tested Applications
ELISA, FC, IF, IHC, WB
Host or Source
Mouse
Preservative
Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
Isotype
IgG2b
Entrez
959
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