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MKI67 Antibody (Biotin)

Mouse monoclonal antibody against MKI67 conjugated to Biotin

Product Specifications

Product Name Alternative

Anti-KI-67 antibody, anti-Ki67 antibody, anti-KI-67 Antigen (KIA) antibody, anti-MKI67 antibody, anti-Proliferation related Ki-67 antigen antibody

UniProt

P46013

Reactivity

Human

Immunogen

Recombinant fragment (around aa 2293-2478) of human Ki67 protein (exact sequence is proprietary)

Target

MKI67

Clonality

Monoclonal

Clone

MKI67/2465

Conjugation

Biotin

Field of Research

Signal Transduction

Purification

Protein A

Concentration

Purified Ab conjugated to Biotin

Dilution

Flow Cytometry (5ul per test per million cells) ; Immunofluorescence (2-4ug/ml at RT) ; , Immunohistochemistry (Formalin-fixed) (2-4ug/ml at RT) ; , (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Form

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.

Molecular Weight

345kDa and 395kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Positive Control: Any actively proliferating cells. Human skin, tonsil or lymph node.

Tested Applications

FC, IF, IHC

Host or Source

Mouse

Preservative

Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.

Isotype

IgG2b

Entrez

4288

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