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PECAM-1 Antibody

Mouse monoclonal antibody to CD31

Product Specifications

Product Name Alternative

EndoCAM; PECA1; Platelet Endothelial Cell Adhesion Molecule 1; GPIIA'

UniProt

P16284

Reactivity

Human

Immunogen

Prokaryotic recombinant fusion protein corresponding to a portion of the extracellular domain downstream of the signal sequence of the CD31 (PECAM-1)

Target

PECAM-1

Clonality

Monoclonal

Clone

1A10

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Concentration

Culture Supernatant

Dilution

Western Blot (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1:50-1:100 for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.

Form

Tissue culture supernatant with 0.05% Azide. Contact us if you require it in a different format.

Molecular Weight

~100kDa (endothelium) and ~130kDa (platelets) '

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Positive Control: THP-1, Jurkat cells. Tonsil or Angiosarcoma.

Tested Applications

IHC, WB

Host or Source

Mouse

Preservative

Tissue culture supernatant with 0.05% Azide. Contact us if you require it in a different format.

Isotype

IgG1

Entrez

5175

Frequently Asked Questions

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