Neurofilament (H+L) (Neuronal Marker) Antibody
Mouse monoclonal antibody to Neurofilament (H+L) (Neuronal Marker)
Product Specifications
Product Name Alternative
NEFH; Neurofilament H; Neurofilament Heavy Polypeptide 200kDa; Neurofilament Triplet H Protein; NF-H; NF200. 68kDa neurofilament protein, Light molecular weight neurofilament protein, NEFL, Neurofilament light polypeptide 68kDa, Neurofilament light polypeptide, Neurofilament protein, light chain, Neurofilament subunit NF-L, Neurofilament triplet L protein, NF-L, NF68
UniProt
P12036
Reactivity
Human, Mouse, Rat
Immunogen
Human NF-H from isolated brain cells
Clonality
Monoclonal
Clone
2F11
Conjugation
Unconjugated
Field of Research
Cardiovascular Research, Neuroscience
Concentration
Purified Ab with BSA and Azide at 200ug/ml
Dilution
Western Blot (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes) ; Optimal dilution for a specific application should be determined.
Form
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Molecular Weight
200kDa & 68kDa
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: IMR-32 cells. Cerebellum or Neuroblastoma
Tested Applications
IHC, WB
Host or Source
Mouse
Isotype
IgG1
Entrez
4744
Available Sizes
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