Cyanine5 DBCO (hexafluorophosphate)

Cyanine5 DBCO (DBCO-Cy5) hexafluorophosphate is a low-toxicity azide reactive probe (NIR fluorescent dye), for imaging azide-labeled biomolecules via a copper-free "click-through" reaction. Cyanine5 DBCO hexafluorophosphate has no apparent cytotoxicity or animal toxicity and shows no damage to the physiological functions of cells other than the target cells (azide-labeled cells) . Cyanine5 DBCO hexafluorophosphate can be used to label and track cells in vitro and in vivo (Ex=635 nm, Em=650-700 nm) [1][2].

Product Specifications

CAS Number

[2360411-64-7]

Product Name Alternative

DBCO-Cy5 (hexafluorophosphate) ; Cyanine5 dibenzocyclooctyne (hexafluorophosphate)

UNSPSC

12171500

Hazard Statement

H302, H315, H319, H335

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/cyanine5-dbco.html

Purity

99.65

Solubility

DMSO : 125 mg/mL (ultrasonic)

Smiles

O=C(CCCCC[N+]1=C(/C=C/C=C/C=C2N(C)C3=C(C\2(C)C)C=CC=C3)C(C)(C)C4=C1C=CC=C4)NCCCCCC(N5C6=C(C#CC7=C(C5)C=CC=C7)C=CC=C6)=O.[F-][P+5]([F-])([F-])([F-])([F-])[F-]

Molecular Formula

C53H59F6N4O2P

Molecular Weight

929.03

Precautions

H302, H315, H319, H335

References & Citations

[3]Song S, et al. In Situ One-Step Fluorescence Labeling Strategy of Exosomes via Bioorthogonal Click Chemistry for Real-Time Exosome Tracking In Vitro and In Vivo. Bioconjug Chem. 2020 May 20;31 (5) :1562-1574. |[1]Kang SW, et al. Cell labeling and tracking method without distorted signals by phagocytosis of macrophages. Theranostics. 2014 Feb 12;4 (4) :420-31. |[2]Zhu L, et al. Coupling Aptamer-based Protein Tagging with Metabolic Glycan Labeling for In Situ Visualization and Biological Function Study of Exosomal Protein-Specific Glycosylation. Angew Chem Int Ed Engl. 2021 Aug 9;60 (33) :18111-18115.