Mouse Nectin-4 Protein, Fc Tag (MALS verified)

Nectin-4 (gene name PVRL4, poliovirus receptor-like 4) is a 66 kDa type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. Nectins are cell adhesion molecules that play a key role in various biological processes such as polarity, proliferation, differentiation and migration, for epithelial, endothelial, immune and neuronal cells, during development and adult life. Nectin-4 is a tumor-associated antigen in 50%, 49% and 86%o of breast, ovarian and lung carcinomas, respectively, mostly on tumors of bad prognosis. Its expression is not detected in the corresponding normal tissues.

Product Specifications

Background

Specifications

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 60.8 kDa. The protein migrates as 65-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q8R007-1

Expression Region

Gly 31 - Ser 347

Host

HEK293

Target

Nectin-4

Conjugation

Unconjugated

Tag

C-Fc

Source

Mouse

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.1 EU per μg

Purity

95%

Bioactivity

Immobilized Human Nectin-1, His Tag (Cat. No. PV1-H5223) at 5 μg/mL (100 μL/well) on an Nickel Coated plate can bind Mouse Nectin-4, Fc Tag (Cat. No. NE4-M5257) with a linear range of 0.156-2.5 μg/mL (QC tested).