KRT14 Antibody (Biotin)
Mouse monoclonal antibody against KRT14 conjugated to Biotin
Product Specifications
Product Name Alternative
Anti-CK-14 antibody, anti-Dowling Meara antibody, anti-ebs3 antibody, anti-ebs4 antibody, anti-Epidermolysis Bullosa Simplex antibody, anti-Keratin Type I Cytoskeletal 14 antibody, anti-Koebner antibody, anti-NFJ antibody
UniProt
P02533
Reactivity
Human
Immunogen
A synthetic peptide of 15 amino acid from the C-terminus of human keratin 14.
Target
KRT14
Clonality
Monoclonal
Clone
LL002
Conjugation
Biotin
Field of Research
Cell Biology, Stem Cell & Developmental Biology
Purification
Protein G
Concentration
Purified Ab conjugated to Biotin
Dilution
Flow Cytometry (2-4ug/million cells) ; Immunofluorescence (2-4ug/ml) ; , Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT), (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Form
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Molecular Weight
~50kDa
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: HeLa, A549, or A431 cells. Skin or squamous cell carcinoma.
Tested Applications
FC, IF, IHC
Host or Source
Mouse
Preservative
Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
Isotype
IgG3
Entrez
3861
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