Pepsin Reagent (ready to use)

1. Deparafinize FFPE tissue section is carried out as outlined in your protocol. Hydrate tissue with PBS or ImmunoBioScience Universal Immuno buffer. 2. Remove buffer, add 2-4 drops of Pepsin reagent to cover tissue section. 3. Incubate at 37oC or at 56oC for 5-10 minutes (the time required for optimum digestion of FFPE tissue section will vary with the extent of fixation, generally approx. 5-10 minutes are sufficient at the above temperatures. In some cases it may require 15 minute.). 4. Discard Pepsin reagent, wash 3-5 times with buffer. 5. The tissue sections are ready for further IHC protocol. NOT RECOMMENDED FOR CYTOKERATIN (clone: AE3) ANTIBODY