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EPCAM Antibody (Biotin)

Mouse monoclonal antibody against EpCAM conjugated to Biotin

Product Specifications

Product Name Alternative

Anti-Adenocarcinoma-associated Antigen antibody, anti-Cell Surface Glycoprotein Trop-1 antibody, anti-EGP2 antibody, anti-EGP314 antibody, anti-EGP40 antibody, anti-Epithelial Cell Adhesion Molecule antibody, anti-Epithelial Glycoprotein 314 antibody, anti-ESA antibody, anti-KSA antibody, anti-TACD1 antibody, anti-TROP1 antibody, anti-Tumor-associated Calcium Signal Transducer 1 (TACSTD1) antibody, anti-ECS-1 antibody, anti-Epidermal Surface Antigen 1 antibody, anti-ESA1 antibody, anti-FLOT2 antibody, anti-Flotillin-2 antibody, anti-Membrane Component antibody, anti-Chromosome 17 antibody, anti-Surface Marker-1 (M17S1) antibody, anti-REG-1 antibody, anti-Reggie-1 antibody, anti-Reggie-2 antibody

UniProt

P16422

Reactivity

Human

Immunogen

Small cell lung carcinoma cells

Target

EPCAM

Clonality

Monoclonal

Clone

VU-1D9

Conjugation

Biotin

Field of Research

Immunology & Inflammation

Purification

Protein G

Concentration

Purified Ab conjugated to Biotin

Dilution

Flow Cytometry (5ul per test per one million cells or 5ul per 100ul of whole blood) ; Immunofluorescence (2-4ug/ml) ; , Immunohistochemistry (Formalin-fixed) (2-4ug/ml at RT) ; , (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Form

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.

Molecular Weight

40-43kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Positive Control: HT29 cells or breast tumor

Tested Applications

FC, IF, IHC

Host or Source

Mouse

Preservative

Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.

Isotype

IgG1

Entrez

4072

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