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TNFRSF9 Antibody (Biotin)

Mouse monoclonal antibody against TNFRSF9 conjugated to Biotin

Product Specifications

Product Name Alternative

Anti-4-1BB Ligand Receptor T Cell antibody, anti-Antigen 4-1BB Homolog antibody, anti-CDw137 antibody, anti-HLDA VI antibody, anti-Homolog of mouse 4 1BB antibody, anti-induced by lymphocyte activation (ILA) antibody, anti-Interleukin activated receptor homolog of mouse Ly63 antibody, anti-T-cell antigen 4-1BB / ILA homolog antibody, anti-Tumor necrosis factor receptor superfamily member 9 antibody

UniProt

Q07011

Reactivity

Human

Immunogen

A recombinant fragment (around aa 19-188) of human CD137 protein (exact sequence is proprietary)

Target

TNFRSF9

Clonality

Monoclonal

Clone

4-1BB/3201

Conjugation

Biotin

Field of Research

Immunology & Inflammation

Purification

Protein G

Concentration

Purified Ab conjugated to Biotin

Dilution

ELISA (For coating, order antibody without BSA) ; , Immunofluorescence (2-4ug/ml) ; Flow Cytometry (2-4ug/million cells) ; Western Blot (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT), (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Form

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.

Molecular Weight

32kDa (monomer) ; 85kDa (dimer)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Positive Control: Human kidney cancer, liver, stomach or thyroid cancer tissue (IHC-P) . Expressed on the surface of activated T-cells.

Tested Applications

ELISA, FC, IF, IHC, WB

Host or Source

Mouse

Preservative

Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.

Isotype

IgG1

Entrez

3604

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