Tau-441 (2N4R) Wild-Type Oligomers

Human Recombinant Tau-441 (2N4R) Wild-Type Oligomers (Baculovirus/Sf9)

Product Specifications

Background

Tau (tubulin-associated unit) is normally located in the axons of neurons where it stabilizes microtubules. Tauopathies such as Alzheimer’s Disease (AD) are characterized by neurofibrillary tangles containing hyper-phosphorylated tau fibrils (1) with consensus that tau oligomers are the most toxic species initiating neurodegeneration (2) . Hyper-phosphorylated tau can be generated via expression in the Sf9/Baculovirus system, with up to 20 sites confirmed by mass spectrometry and western blots with phospho-specific antibodies (3) . Our Sf9/Baculovirus-expressed Tau 2N4R oligomers are formed without the use of heparin or another anionic scaffold.

Product Name Alternative

MAPT, intracellular neurofibrillary tangles, NFTs, paired helical filaments, PHFs, 2N4R

UNSPSC

12352202

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Swiss Prot

P10636-8

Expression System

Baculovirus/Sf9

Host

Baculovirus/sf9

Origin Species

Human

Target

Wild-Type Tau

Conjugation

No Tag

Nature

Recombinant

Sequence

MAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQTPTEDGSEEPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAAQPHTEIPEGTTAEEAGIGDTPSLEDEAAGHVTQARMVSKSKDGTGSDDKKAKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL

Applications

WB | SDS-PAGE | Native-PAGE | In vivo assay | In vitro assay

Field of Research

Neuroscience | Neurodegeneration | Alzheimer's Disease | Tangles & Tau

Purification Method

Ion-exchange Purified

Purification

Ion-exchange Purified

Limit Of Detection

Certified >95% pure using SDS-PAGE and A260/A280 analysis. Low endotoxin <5 EU/mL @ 2mg/mL.

Concentration

2 mg/mL

Purity

>95%

Weight

0.05

Length

Full Length (1-441 aa)

Buffer

PB pH 7.4

Molecular Weight

45.84 kDa

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For research use only.

Additionnal Information

Monomer source is catalog# SPR-496. For corresponding PFFs, see catalog# SPR-498.

References & Citations

1. Iqbal K., Liu F., and Gong C.X. 2016. Tau and neurodegenerative disease: The story so far. Nat. Rev. Neurol. DOI: 10.1038/nrneurol.2015.225 2. Puangmalai, N., Bhatt, N., Montalbano, M. et al. 2020. Internalization mechanisms of brain-derived tau oligomers from patients with Alzheimer’s disease, progressive supranuclear palsy and dementia with Lewy bodies. Cell Death Dis. DOI: 10.1038/s41419-020-2503-3 3. Tepper et al. 2014. Oligomer Formation of Tau Protein Hyperphophorylated in Cells. The Journal of Biological Chemistry, DOI: 10.1074/jbc.M114.611368