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IL-12, Human (Biotinylated, HEK293, His)

IL-12 beta Protein, also known as natural killer cell stimulatory factor 2, is a common subunit (p40) of IL-12 and IL-23. IL-12 is a inflammatory factor expressed by activated macrophages, and involves in Th1-type immune response against cancer[1]. IL-12 beta Protein located outside the cell membrane, involves in singalling mediated by Jak-STAT[3]. IL-12 beta Protein consists of 328 amino acids (M1-S328) with a fibronectin type-III domain (237-328 a.a) . IL-12 beta Protein, Human (M1-S219) is a biotinylated protein, produced in HEK293 cells with a C-Terminal His-tag.

Product Specifications

Product Name Alternative

IL-12 Protein, Human (Biotinylated, HEK293, His), Human, HEK293

UNSPSC

12352202

Type

Recombinant Proteins

Assay Protocol

https://www.medchemexpress.com/cytokines/il-12-alpha-protein-human-biotinylated-hek293-his.html

Molecular Formula

3592&3593 (Gene_ID) P29459 (R23-S219) &P29460 (I23-S328) (Accession)

Molecular Weight

Approximately 24&36.2 kDa.

References & Citations

[1]Ivy M Dambuza, et al. IL-12p35 induces expansion of IL-10 and IL-35-expressing regulatory B cells and ameliorates autoimmune disease. Nat Commun. 2017 Sep 28;8 (1) :719.|[2]Lu X. Impact of IL-12 in Cancer. Curr Cancer Drug Targets. 2017;17 (8) :682-697.|[3]Jin Kyeong Choi, et al. IL-12p35 Inhibits Neuroinflammation and Ameliorates Autoimmune Encephalomyelitis. Front Immunol. 2017 Oct 5;8:1258.|[4]Gunsten S, et al. IL-12 p80-dependent macrophage recruitment primes the host for increased survival following a lethal respiratory viral infection. Immunology. 2009 Apr;126 (4) :500-13.|[5]D De Wit, et al. Helper T-cell responses elicited by Der p 1-pulsed dendritic cells and recombinant IL-12 in atopic and healthy subjects. J Allergy Clin Immunol. 2000 Feb;105 (2 Pt 1) :346-52.|[6]Gotthardt D, Trifinopoulos J, Sexl V, Putz EM. JAK/STAT Cytokine Signaling at the Crossroad of NK Cell Development and Maturation. Front Immunol. 2019 Nov 12;10:2590.|[7]Tait Wojno ED, Hunter CA, Stumhofer JS. The Immunobiology of the Interleukin-12 Family: Room for Discovery. Immunity. 2019 Apr 16;50 (4) :851-870.|[8]Becker-Hapak MK, et al. A Fusion Protein Complex that Combines IL-12, IL-15, and IL-18 Signaling to Induce Memory-Like NK Cells for Cancer Immunotherapy. Cancer Immunol Res. 2021 Sep;9 (9) :1071-1087.|[9]Krämer B, et al. Do lambda-IFNs IL28A and IL28B act on human natural killer cells? Proc Natl Acad Sci U S A. 2011 Aug 23;108 (34) :E519-20; author reply E521-2.

Shipping Conditions

Dry ice.

Scientific Category

Recombinant Proteins

Frequently Asked Questions

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