Mouse anti Integrin beta 1D

Integrins are a family of heterodimeric membrane glycoproteins consisting of non-covalently associated α and β subunits. More than 18 α and 8 β subunits with numerous splice variant isoforms have been identified in mammals. In general, integrins function as receptors for extracellular matrix proteins. Certain integrins can also bind to soluble ligands or to counter-receptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), resulting in aggregation of cells. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migRation and apoptosis. There are two major forms of integrin β1: β1A and β1D, which differ in 13 amino acids. Their distribution pattern in adult tissues is mutually exclusive. β1A is present in all tissues, except cardiac and skeletal muscle, which instead express the β1D variant.

1G2 is a Mouse monoclonal IgG2a, κ antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a Mouse immunized with a synthetic peptide corresponding to the C-terminal 24 amino acids of integrin β1D including an appending N-terminal cysteine (CQENPIYKS-PINNFKNPNYGRKAGL) coupled to keyhole limpet hemocyanin.

1G2 is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration; recommended range is 1:50 – 1:100 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:500 for immunoblotting applications.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

1. van der Flier, A., Gaspar, A. C., Thorsteinsdottir, S., Baudoin, C., Groeneveld, E., Mummery, C. L., and Sonnenberg, A. (1997). Spatial and temporal expression of the beta1D integrin during Mouse development, Dev Dyn 210, 472-86.

The antibody is shipped at ambient temperature and may be stored at +4°C; For prolonged storage prepare appropriate aliquots and store at or below -20°C; Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2); Repeated thawing and freezing should be avoided; Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day; If a slight precipitation occurs upon storage, this should be removed by centrifugation; It will not affect the performance or the concentration of the product