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His tag, AlpSdAbs® VHH (Biotin)

Anti-His tag, AlpSdAbs® VHH (Biotin) is designed for detecting His tag fusion proteins. Anti-His tag, AlpSdAbs® VHH (Biotin) is based on monoclonal, recombinant, single domain antibody to His tag coupled to Biotin, and Anti-His tag, AlpSdAbs® VHH (Biotin) detects the His tag selectively, no reactivity with other proteins., . . . .

Product Specifications

Host

Alpaca

Immunogen

6*His tag fusion protein

Conjugation

Biotin-conjugated

Type

Alpaca monoclonal Antibody

Applications

ELISA, Purification

Concentration

1 mg/mL

Dilution

WB: 1:5,000-1:20000 ;ELISA: 1:5,000-1:20000 ;IP: 1-2ug/sample,BLI (biolayer interferometry),SPR (surface plasmon resonance), ;Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Purity

Recombinant Expression and Affinity purified

Buffer

10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300

Storage Conditions

Store at –20°C (Avoid freeze / thaw cycles)

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