His tag, AlpSdAbs® VHH (Biotin)
Anti-His tag, AlpSdAbs® VHH (Biotin) is designed for detecting His tag fusion proteins. Anti-His tag, AlpSdAbs® VHH (Biotin) is based on monoclonal, recombinant, single domain antibody to His tag coupled to Biotin, and Anti-His tag, AlpSdAbs® VHH (Biotin) detects the His tag selectively, no reactivity with other proteins., . . . .
Product Specifications
Host
Alpaca
Immunogen
6*His tag fusion protein
Conjugation
Biotin-conjugated
Type
Alpaca monoclonal Antibody
Applications
ELISA, Purification
Concentration
1 mg/mL
Dilution
WB: 1:5,000-1:20000
;ELISA: 1:5,000-1:20000
;IP: 1-2ug/sample,BLI (biolayer interferometry),SPR (surface plasmon resonance),
;Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Purity
Recombinant Expression and Affinity purified
Buffer
10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage Conditions
Store at –20°C (Avoid freeze / thaw cycles)
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