Anti-CD73/5'Nucleotidase Antibody

Mouse Monoclonal Antibody specific to CD73/5'-Nucleotidase

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

5'-NT, EC 3.1.3.5, Ecto-5'-nucleotidase, CD antigen CD73

Gene Name

NT5E

NCBI Gene ID

UniProt

P21589

Cellular Locus

Cell membrane; Lipid-anchor, GPI-anchor.

Host

Mouse

Reactivity

Human

Immunogen

Recombinant 5'-nucleotidase (5'-NT). Accession no. P21589.

Target Antigen

5'-nucleotidase

Target

CD73/5'Nucleotidase

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC, ELISA

Field of Research

Enzymes

Purification Method

Purified by Protein A affinity chromatography

Dilution

Reconstitute lyophilized antibody using ultrapure to a final concentration no less than 100 ug/mL. Reconstitution with PBS or Tris buffer is acceptable if required. Protein carrier, biocide or cryopreservative can be added as needed. Aliquot and store at -20C for longterm storage. Dilute immediately prior to use.

Format

Purified

Form

Lyophilized

Buffer

Tris Buffer

Function

Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities. {PubMed:21933152}.

Additionnal Information

ELISA: use at 1-10ug/ml Immunoblotting: User should use at 1-5ug/ml. A band of ~63kDa is detected. Immunohistochemistry: use at 1-5ug/ml. These are recommended concentrations. End user should determine optimal concentrations for their applications.

Storage Conditions

This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.

Specificity

This antibody reacts with human 5'-NT.

Formulation

Lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose, 1mg/ml.

Target Background

5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (Cluster of Differentiation 73), is an enzyme that in humans is encoded by the NT5E gene. CD73 converts AMP to adenosine. This enzyme consists of a dimer of 2 identical 70-kDa subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. A deficiency of NT5 occurs in a variety of immunodeficiency diseases