Mouse IL-12/23 (P40) ELISA Kit
Product Specifications
Reproducibility
The coefficient of variation of both intra-assay and inter-assay were less than 10%.
CAS Number
7732-18-5
Gene Name
Tumor necrosis factor
Gene Aliases
DIF, TNFA, TNFSF2, TNLG1F, TNF-alpha
Gene ID
7124
Reactivity
Human
Target
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
Type
ELISA Kit
Applications
ELISA
Detection Method
Colorimetric, OD450 nm
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Cell culture, supernates, serum, and plasma
Sensitivity
7 pg/ml
Homology
Human
Components
Components 2 vials human TNF-alpha Standard lyophilized, 4000 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human TNF-alpha antibody 2 vials Streptavidin-HRP solution, 1 bottle Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Stop Solution 2 vials human TSLP Standard lyophilized, 500 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human TSLP monoclonal antibody Components Components 2 vials human TNF-alpha Standard lyophilized, 4000 pg/ml upon reconstitution 2 vials human TNF-alpha Standard lyophilized, 4000 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human TNF-alpha antibody 2 vials concentrated Biotin-Conjugate anti-human TNF-alpha antibody 2 vials Streptavidin-HRP solution, 2 vials Streptavidin-HRP solution, 1 bottle Standard /sample Diluent 1 bottle Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Substrate Solution 1 vial Stop Solution 1 vial Stop Solution 2 vials human TSLP Standard lyophilized, 500 pg/ml upon reconstitution 2 vials human TSLP Standard lyophilized, 500 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human TSLP monoclonal antibody 2 vials concentrated Biotin-Conjugate anti-human TSLP monoclonal antibody
NCBI Gene Symbol
TNF
Gene Name URL
TNF
Nucleotide Accession Number
NM_000594.3
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