TNF ELISA Kit (Human)
Product Specifications
Reproducibility
The coefficient of variation of both intra-assay and inter-assay were less than 10%.
CAS Number
7732-18-5
Gene Name
Matrix metallopeptidase 9
Gene Aliases
GELB, CLG4B, MMP-9, MANDP2
Gene ID
4318
Reactivity
Human
Target
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
Type
ELISA Kit
Applications
ELISA
Detection Method
Colorimetric, OD450 nm
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Cell culture, supernates, serum, and plasma
Sensitivity
31 pg/ml
Homology
Human
Components
Components 2 vials human MMP-9 Standard lyophilized, 4000 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human MMP-9 antibody 2 vials Streptavidin-HRP solution, 1 bottle Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Stop Solution 2 vials human sICAM-1 Standard lyophilized, 4000 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human sICAM-1 antibody Components Components 2 vials human MMP-9 Standard lyophilized, 4000 pg/ml upon reconstitution 2 vials human MMP-9 Standard lyophilized, 4000 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human MMP-9 antibody 2 vials concentrated Biotin-Conjugate anti-human MMP-9 antibody 2 vials Streptavidin-HRP solution, 2 vials Streptavidin-HRP solution, 1 bottle Standard /sample Diluent 1 bottle Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Substrate Solution 1 vial Stop Solution 1 vial Stop Solution 2 vials human sICAM-1 Standard lyophilized, 4000 pg/ml upon reconstitution 2 vials human sICAM-1 Standard lyophilized, 4000 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human sICAM-1 antibody 2 vials concentrated Biotin-Conjugate anti-human sICAM-1 antibody
NCBI Gene Symbol
MMP9
Gene Name URL
MMP9
Nucleotide Accession Number
NM_004994.2
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